Fig. 5: Failed nuclear export of pre-60S particles.
From: Nuclear export of the pre-60S ribosomal subunit through single nuclear pores observed in real time
a The bar height indicates the frequency of the observation of the respective dwell times. The total number of evaluated trajectories was n = 145. The blue line indicates the best fit using a single exponential decay function and yielded τ = 13 ± 3 ms. b (Top) The trajectories were aligned to the transporting NPC (as indicated by the maximum of the eGFP-NTF2 signal) as the origin and then rotated such that the export direction coincided with the positive x axis. (Bottom) The number of pre-60S particle positions in a y axis region ±100 nm off the central NPC was plotted as a function of the location with regard to the average NTF2 accumulation. The maximum clearly coincides with the nuclear basket. The total number of single positions shown is n = 788.
