Fig. 5: Kinetics of phot1-mediated phosphorylation of NPH3. | Nature Communications

Fig. 5: Kinetics of phot1-mediated phosphorylation of NPH3.

From: Regulation of plant phototropic growth by NPH3/RPT2-like substrate phosphorylation and 14-3-3 binding

Fig. 5

a Time-course of S744 phosphorylation. Immunoblot analysis of total protein extracts from etiolated wild-type seedlings irradiated with 0.5 µmol m−2 s−1 of blue light for the time indicated. Blots were probed with anti-pS744 and anti-NPH3 antibodies. b Time-course of S744 dephosphorylation. Immunoblot analysis of total protein extracts from etiolated wild-type seedlings maintained in darkness (D) or irradiated with 0.5 µmol m−2 s−1 for 15 min and returned to darkness for the time indicated. Blots were probed with anti-pS744 and anti-NPH3 antibodies. Experiments were repeated at least twice with similar results.

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