Fig. 3: Itgβ8 expression in Tregs impairs cytotoxic functions of tumor CD8pos T cells.
From: Regulatory T cells promote cancer immune-escape through integrin αvβ8-mediated TGF-β activation
a, b Foxp3Ctrl mice, in black, and Foxp3ΔItgβ8 animals, in red, were treated or not with depleting anti-CD8β to selectively remove CD8pos T cells. Animals were then injected i.d. with B16 cells. Pictures of tumors are representative of 6–11 animals from two independent experiments and the graph represents the tumor size at day 15 after tumor implantation. c–f Foxp3ΔItgβ8 mice and their littermate controls (Foxp3Ctrl) were injected either i.d. with B16 cells and tumors and their draining lymph node (dtLN) were analyzed by flow cytometry 15 days later (c, f). Counterplots illustrate the ability of CD8 cells to produce granzyme B (GzB) and degranulate based on CD107 cell surface expression CD8pos T cells infiltrating the tumor. Percentages of the gated populations are mentioned on counterplots. Histograms demonstrate the percentage of CD8pos T cells expressing GzB or co-expressing GzB and CD107 in different animals (n = 7) from two independent. d, e Representative pictures demonstrate TUNEL-positive cells (yellow nucleus), tumor slides were stained with DAPI (blue). The graph illustrates the density of apoptotic cells. Data are representative of two experiments, with 7–8 mice per group. Error bar, mean ± SD. For b, c, f, data are presented as mean ± SD. b P = 0.0422 (*)/P = 0.4771 (ns), c P = 0.0012 (**)/P = 0.0175 (*), e P = 0.0293. **P < 0.01, *P < 0.05. The paired two-tailed Student t test for b, Unpaired two-tailed Student t test for c, two-tailed Mann–Whitney for c–f. Scale bar: 50 µm. Source data are provided as a Source Data file.
