Fig. 1: Development of RADAR.
From: Engineering digitizer circuits for chemical and genetic screens in human cells
A Schematic of how RADAR functions. The primarily tested inducible promoter in this study was sensitive to AP-1, though the system is also shown to be compatible with other promoters (WNT, NF-kB, or CAGA12). B Construct comparison between RADAR and a classical reporter. C Regulation of reporter-driven GFP expression using combinations of ABA, PMA, and DMSO (vehicle control) (n = 3, mean ± s.d.). D Signal-to-noise ratios of RADAR and classical reporters when comparing “on” (ABA/PMA for RADAR, PMA for classical) and “off” (ABA for RADAR, DMSO for classical) signals (n = 3, mean ± s.d.). E Retainment of GFP expression over time with comparison between RADAR and classical reporters (n = 3, mean ± s.d.). F Dose response of RADAR measured after 1, 2, 3, and 4 days’ of incubation in ABA and PMA (n = 3, mean ± s.d.). G Sensitivity of RADAR to a low dose of PMA (0.4 ng/ml) over time with an increasing percentage of cells switching “on”. H Application of RADAR to other pathway-sensitive promoters.
