Fig. 2: Application of RADAR to compound screening.
From: Engineering digitizer circuits for chemical and genetic screens in human cells
A Schematic of the compound screening process. B Maximum and minimum luciferase levels attained by each reporter for each compound in the agonist and antagonist reconfirmation screens, respectively. RLU values were normalized to the median of the negative controls. RADAR data is depicted in green, and classical reporter data depicted in blue. C Examples of compound hits found by RADAR that were missed by the classical reporter in agonist and antagonist reconfirmation screens (compounds C3166 and C3394, respectively). All RLU values were normalized to the median of the negative controls (n = 2). D AC50 values of shared agonist hits between both reporters in the reconfirmation screen. E Accumulation of specific compound hits in the agonist reconfirmation screen with 40,000 cells/ml cell density over time.
