Fig. 5: Muscle development restricts microtubule-independent mRNA dispersion.
From: Microtubule-based transport is essential to distribute RNA and nascent protein in skeletal muscle
A Polr2a FISH in C2C12 myoblast after 6 h control (DMSO) culture. Scale bars: 2 µm. B Polr2a FISH in C2C12 myoblast after 6 h culture with nocodazole. Scale bars: 2 µm. C Polr2a FISH in C2C12 myotube after 6 h control (DMSO) culture. Scale bars: 2 µm. D Polr2a FISH in C2C12 myotube after 6 h culture with nocodazole. Scale bars: 2 µm. E Polr2a FISH in myofiber after 6 h control (DMSO) culture. Scale bars: 2 µm. F Polr2a FISH in myofiber after 6 hr culture with nocodazole. Scale bars: 2 µm. G Percent dispersion for Polr2a RNA in C2C12 myoblasts, C2C12 myotubes, and isolated myofibers treated with nocodazole or control (DMSO) for 6 h. Black lines are medians. n = 5 myotubes and myofibers per condition. n = 10 and 12 cells for nocodazole and DMSO-treated myoblasts, respectively. *p < 0.05, **p < 0.01, Two-sided Mann–Whitney U test.
