Fig. 7: Regulators of RNP granule formation, transport, and translation co-accumulate at the nuclear periphery of nocodazole-treated myofibers.
From: Microtubule-based transport is essential to distribute RNA and nascent protein in skeletal muscle
A Representative images of Co-IF against the nuclear pore complex (NPC, labeled with mAb 414) and Kif1C in myofibers cultured for 18 h in the presence of nocodazole or control (DMSO). Scale bars: 2 µm. B Representative images of Co-IF against G3bp1 and Fxr1p in myofibers cultured for 18 h in the presence of nocodazole or control (DMSO). See also Supplementary Fig. 6A, B. Scale bars: 2 µm. C Representative images of Co-IF against the Mbnl1 and Tdp-43 in myofibers cultured for 18 h in the presence of nocodazole or control (DMSO). Scale bars: 2 µm. D Perinuclear to cytoplasmic relative IF signal density in nocodazole (red) and control (DMSO, blue) myofibers. n = 5 myofibers per protein/condition. *p < 0.05, Two-sided Mann–Whitney U test.
