Fig. 8: RNAs exhibit restricted diffusion and directed transport in myotubes.

A Schematic describing MS2 live-cell RNA imaging strategy. B Example image of a myotube expressing MS2-labeled RNA (top) and RNA trajectories (bottom) from a 53-s imaging time course. Scale bars: 2 µm. C Percentage of RNA trajectories in each motion category. D Diffusion coefficients for low-mobility (red) and high-mobility (green) diffusive tracks and example tracks from both groups. X-axis ticks for scale: 0.5 µm. E Distance and velocity for processive (blue) and crawling trajectories (purple) along with example tracks from both groups. X-axis ticks for scale: 0.5 µm. F Series of images showing RNA particle splitting and merging events. Colored triangles denote particle identities. Scale bars: 1 µm. G Example RNA tracks from a myotube expressing MS2-labeled RNA during a 50-min imaging time course. Scale bars: 2 µm. H Maximum distance traveled for each track from the 50-min (red) and 2-min (blue) imaging time courses. Tracks below dotted line (1 µm) are categorized as stationary. I Fraction of stationary tracks in 50-min and 2-min time courses.