Fig. 1: Characterization of binding between different RBDs and hACE2 using flow cytometry. | Nature Communications

Fig. 1: Characterization of binding between different RBDs and hACE2 using flow cytometry.

From: Molecular insights into receptor binding of recent emerging SARS-CoV-2 variants

Fig. 1

BHK cells stably expressing GFP and hACE2 were incubated with His-tagged MERS-CoV RBD, SARS-CoV-2 WT RBD, Alpha RBD, Beta RBD, Gamma RBD, Mink-Y453F RBD, Mink-N501T RBD, and Mink-F486L RBD, respectively. APC anti-His antibodies were used to detect the His-tagged protein binding to the cells. Representative results from three experiments are shown. The mean ± SD percentages of RBD-binding cells in the three experiments are shown in the right lower bar chart. Statistical significance was analyzed using one-way ANOVA with a Tukey’s multiple comparison test for multiple groups.

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