Fig. 9: Elevated 20S proteasome levels (Hi20S cells) facilitate survival under proteotoxic stress.
From: The 20S as a stand-alone proteasome in cells can degrade the ubiquitin tag
a Phase contrast image of culture plate showing survival of control and Hi20S cells after 36 h of puromycin treatment (6 μg/mL). b Cell survival at increasing puromycin exposure. Control and Hi20S cells were grown for 36 h in the presence of increasing puromycin concentrations. Cell survival was quantified by the MTT assay. Data represent the average of three experimental values (±SD error bar). c Control and Hi20S cells were treated with puromycin (5 µg/mL) for 2 h and then recovered over 12 h. Cell lysates were subjected to IB with anti-puromycin antibody. d HeLa cells were grown under hypoxia and treated with puromycin as described in (c) IB was performed with anti-puromycin antibody. e The rate of puromycylated polypeptides removal under normoxia and hypoxia. Data represent the average percentage of puromycin-containing polypeptides (±SD error bar) at each time point of the recovery phase, quantified from IB of three independent experiments. f Cell survival upon puromycin exposure during hypoxia. HeLa cells were grown under normoxia or hypoxia with puromycin (250 ng/mL) for up to 60 h. Cell survival was quantified by the MTT assay. Data represent the average of three experimental values (±SD error bar). Source data are provided as a Source data file.
