Fig. 2: Structure of the MACV L–Z complex.
From: Structure of Machupo virus polymerase in complex with matrix protein Z
a Atom model of the L–Z complex shown as ribbon in two orientations. The domains are indicated and colored as in Fig. 1a. b Superposition of MACV L in the states of apo (colored gray) and Z-bound monomer (colored as Fig. 1a). c Structure of Z in the L–Z complex shown as ribbon and colored orange, with two divalent zinc atom shown as gray spheres. Side chains of conserved cysteines and histidine involved in Zn binding are shown as sticks and labeled. Although full-length MBP-Z fusion protein is used for structural study, only residues 34–82 are visible in the complex. d Sequence alignment for RING domain between the MACV and LASV Z proteins. Residues involved in the interaction with the L protein are marked below with black solid triangle, while conserved cysteines and histidine involved in Zn binding are marked below with green italicized C or H. Secondary structural elements are depicted on the top of the alignments.
