Fig. 1: Characterization of the fibrils formed by G51D α-syn.
From: The hereditary mutation G51D unlocks a distinct fibril strain transmissible to wild-type α-synuclein
a ThT kinetic assay of the G51D fibril formation with (colored in dark green) and without PFFs (5 mol%) (colored in light green) in PB Buffer (50 mM phosphate buffer, pH 7.0, 50 mM NaCl). Data are shown as mean ± s.d., n = 5 independent samples (left panel). The fibrils formed at the endpoint of the ThT kinetic assay were characterized by NS-TEM (right panel). Scale bar: 200 nm. b Histogram of half-pitch lengths of G51D fibrils (0% PFFs) measured from NS-TEM images (n = 83). c AFM image of a G51D fibril. The arrows at both sides of the fibril indicate the starting points of the fibril protrusions to clarify the handedness. Images were acquired with a 1.45 Hz rate at 512 × 512 pixels. Fibrils formed by G51D in >3 independent experiments provide reproducible images. The fibril width and periodicity were analyzed by the Nanoscope software.
