Fig. 4: Seeding of WT α-syn by both G51D and WT α-syn PFFs.
From: The hereditary mutation G51D unlocks a distinct fibril strain transmissible to wild-type α-synuclein
a Schematic diagram of the seeding process. PDB IDs of WT1a and G51D fibrils used for the seeding experiments are provided in parentheses. Buffer (50 mM Tris, pH 7.5, 150 mM KCl, 0.05% NaN3) is referred to as Tris buffer. Buffer (50 mM phosphate buffer, pH 7.0, 50 mM NaCl, 0.05% NaN3 buffer) is referred to as PB buffer. The schematic diagram was created from scratch. b Fibrillation of WT α-syn alone (colored in black) or in the presence of 5 mol% WT PFFs (colored in purple) and G51D PFFs (colored in cyan), respectively. Data are shown as mean ± s.d., n = 5 independent samples. The fibrils sampled at the end of the ThT kinetics assay were imaged by NS-TEM (right panel). Scale bar: 200 nm. c and d AFM images of the WT51cs (c) and WTwts fibrils (d). The images represent reproducible results in three independent experiments. The arrows at both sides of the fibril indicate the starting points of the fibril protrusions to clarify the handedness. Images were acquired with a 1.45 Hz rate at 512 × 512 pixels. The fibril width and periodicity were analyzed by the Nanoscope software.
