Fig. 5: Cryo-EM study of WT51cs and WTwts strains.
From: The hereditary mutation G51D unlocks a distinct fibril strain transmissible to wild-type α-synuclein
Cryo-EM micrographs of WT51cs strain (a) and WTwts strain (b) are shown. The images represent reproducible results in three independent experiments. Scale bar = 100 nm. 686-pixel box size 2D class averages comprising a helical crossover of WT51cs strain (c) and WTwts strain (d) are shown and used to determine crossover distance. e Central slice of the 3D map of the WT51cs and G51D strains. The additional electron density of the WT51cs strain compared to the G51D strain is circled. f Central slice of the 3D map of the WTwts (left). Structural models of WT1a strain (PDB ID: 6A6B) are shown on the right. The topology diagram of α-syn for each strain is shown (bottom).
