Table 1 Statistics of cryo-EM data collection and refinement.

PDB

7E0F

n/a

n/a

EMDB

EMD-30931

n/a

n/a

Magnification

22,500

22,500

22,500

Pixel size (Å)

1.06

1.06

1.06

Defocus range (μm)

−1 to −2

−1 to −2

−1 to −2

Voltage (kV)

300

300

300

Detector

K3

K3

K3

Microscope

Titan Krios

Titan Krios

Titan Krios

Exposure time (s per frame)

0.097

0.097

0.097

Number of frames

32

32

32

Total dose (e− per Ų)

55

55

55

Micrographs

1869

725

1056

Manually picked fibrils

28,988

12,428

7903

Box size (pixel)

288

686

686

Inter-box distance (Å)

30.5

72.7

72.7

Initial particle images (no.)

349,716

90,963

74,598

Final particle images (no.)

213,348

40,587

16,120

Map resolution (Å)

2.96

n/a

n/a

FSC threshold

0.143

n/a

n/a

Map resolution (Å)

3.15

n/a

n/a

FSC threshold

0.5

n/a

n/a

Map sharpening B-factor (Ų)

−144.573

n/a

n/a

Helical rise (Å)

2.43

n/a

n/a

Helical twist (°)

−179.37

n/a

n/a

Initial model used

6L4S

n/a

n/a

non-hydrogen atoms

2, 022

n/a

n/a

Protein residues

294

n/a

n/a

Ligands

0

n/a

n/a

r.m.s.d. bond lengths

0.016

n/a

n/a

r.m.s.d. bond angles

0.986

n/a

n/a

All-atom clash score

9.76

n/a

n/a

Rotamer outliers

0.00%

n/a

n/a

Ramachandran outliers

0.00%

n/a

n/a

Ramachandran allowed

12.77%

n/a

n/a

Ramachandran favored

87.23%

n/a

n/a