Fig. 10: Karst is required in ensheathing glia for normal locomotor behavior. | Nature Communications

Fig. 10: Karst is required in ensheathing glia for normal locomotor behavior.

From: Drosophila ßHeavy-Spectrin is required in polarized ensheathing glia that form a diffusion-barrier around the neuropil

Fig. 10

a Exemplary locomotion tracks of control wandering third instar larvae with the genotype [83E12-Gal4AD, repo-Gal4DBD, UAS-GFPdsRNA]. Larvae move in long run phases. b Upon kst knockdown in ensheathing glia (EG) only [83E12-Gal4AD, repo-Gal4DBD, UAS-kstdsRNA], larvae do not explore the tracking arena. c The insertion of a Trojan Gal4 element into the first coding exon of kst-RE(RG) generates an PE and PG isoform specific mutant (see Fig. 8). Larvae with the genotype [kstTrojanGal4 / Df(3 L)ED208] show a comparable locomotion phenotype as RNAi knockdown larvae. d Locomotion phenotype of zygotic karst null mutant larvae with the genotype [kstMiMIC13613 / Df(3 L)ED208]. e Locomotion phenotype observed following specific ablation of the ensheathing glia [UAS-rpr; 83E12-Gal4AD; repo-Gal4DBD, UAS-hid]. f–h Quantification of peristalsis efficiency, velocity and bending distribution between control larvae, kst knockdown and ensheathing glia ablated animals. Box plots show median (line), boxes represent the first and third percentiles, whiskers show standard deviation, diamonds indicate outliers. i–k Quantification of the same parameters comparing wild type Canton S with kst null larvae and a kstTrojan control strain that carries the Trojan insertion in a non-productive orientation [kstMiMIC03134-Trojan-C in trans to the Df(3 L)ED208] with the isoform specific kstPE, PG mutant [kstMiMIC03134-TrojanGal4 / Df(3 L)ED208. Quantification Wilcoxon rank-sum test, two tailed, n = 50. Peristalsis Efficiency [mm/wave]: GFPdsRNA – kstdsRNA: ****p = 2.65E-32 GFPdsRNA – ablation; ****p = 3.22E-30; velocity [mm/sec]: GFPdsRNA – kstdsRNA: **** p = 3.81E-11; GFPdsRNA – ablation: ****p = 1.54E-05; Peristalsis Efficiency [mm/wave]: Canton S – kstMiMIC / Df: ****p = 3.98E-20; Canton S – Trojan C/ Df: ****p = 4.61E-13; TrojanC – TrojanGal4 / Df: ****p = 1.22E-13; velocity [mm/sec]: Canton S – kstMiMIC / Df: ****p = 1.15E-06; Canton S – TrojanC/ Df: ****p = 1.21E-03; TrojanC – TrojanGal4 / Df: ****p = 5.94E-13. Source data are provided as a Source Data file.

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