Fig. 5: Ensheathing glial cells form an internal barrier around the neuropil. | Nature Communications

Fig. 5: Ensheathing glial cells form an internal barrier around the neuropil.

From: Drosophila ßHeavy-Spectrin is required in polarized ensheathing glia that form a diffusion-barrier around the neuropil

Fig. 5

a Schematic depiction of the dye injection experiments. Fluorescently labelled 10 kDa dextran was injected into the left hemisphere of a wandering third instar larva. Diffusion of the dye was monitored at two positions. In the neuropil (N) and in the cortex (C) harboring all neuronal cell bodies. b, b’ Stills of a representative movie of a larval brain lobe with the genotype [n = 5; 83E12-Gal4, UAS-CD8::GFP] following dye injection, time is indicated. c Quantification of dye diffusion rate in larvae of the genotype [83E12-Gal4, UAS-CD8::GFP] (blue line) and [UAS-rpr; 83E12-Gal4AD, repo-Gal4DBD, UAS-hid] (red line) (n = 5, standard deviation is shown, *p = 0.0181; unpaired t-test, two-tailed). d, d’ Stills of a representative movie of a larval brain lobe with the genotype [n = 5, UAS-rpr; 83E12-Gal4AD; repo-Gal4DBD, UAS-hid] following dye injection. Scale bars are 50 µm. Source data are provided as a Source Data file.

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