Fig. 1: SpCas12f1 and AsCas12f1 CRISPR-Cas loci and effector complex components.
From: Miniature type V-F CRISPR-Cas nucleases enable targeted DNA modification in cells
a Schematic representation of native CRISPR-Cas loci encoding SpCas12f1 and AsCas12f1 effector proteins. b Workflow of the biochemical approach used to isolate and identify Cas12f1 RNP-bound RNA molecules. c In silico prediction of base pairing between identified tracrRNA and crRNA sequences. d Size comparison (aa) of Cas effector proteins (bars) and respective guide RNA length (red line) (nt) with SpCas12f1 and AsCas12f1 nucleases highlighted in the gray area.
