Fig. 3: Optn deficient mice are resistant to EAE pathogenesis and inflammation.
a Western blotting analysis for the expression of OPTN in the BMDCs from four pairs of C57BL/6 Ctrl and EAE mice (8-week-old, female) at day 20 after immunization. n = 3 independent experiments. b Mean clinical scores of Ctrl and OptnΔCD11c mice (8-week-old, female) subjected to MOG35–55-induced EAE. n = 8 independent animals, P = 3.3E−09. c (Upper) Luxolfast blue (LFB) staining of mouse spinal cords from Ctrl and OptnΔCD11c EAE mice at day 20 post immunization (dpi 20). Scale bars, 200 μm. (Lower) Immunofluorescent labeling of CC1 (green) and DAPI (blue) in the spinal cord of indicated mice at day 20 after immunization. Scale bars, 20 μm. n = 3 independent animals. d The summary graphs of CC1+ cells in (c). n = 3 independent animals, P = 0.0004. e, f Frequency of Th1, Th17, Th2, and Treg cells in the spleen (SP) (e) and draining lymph nodes (DLN) (f) of MOG35–55-immunized Ctrl and OptnΔCD11c mice on dpi 20. n = 3 independent animals, P(IFN-γ) = 0.0016, P(IL-17) = 0.0001, P(IL-4) = 0.0082, P(Foxp3) = 0.0051 in (e); P(IFN-γ) = 0.0003, P(IL-17) = 0.0075, P(IL-4) = 0.0103, P(Foxp3) = 0.0088 in (f). g The total numbers of MNCs in whole spinal cord and brain were isolated from Ctrl and OptnΔCD11c EAE mice on dpi 20. n = 3 independent animals, P = 0.0015. Data are presented as means ± SD. *P < 0.05; **P < 0.01; ***P < 0.001. Unpaired two-tailed Student’s t-test. Source data are provided in Source data file.
