Fig. 6: OPTN restrains the IL-6/IL-10 activated JAK2/STAT3 phosphorylation in DCs. | Nature Communications

Fig. 6: OPTN restrains the IL-6/IL-10 activated JAK2/STAT3 phosphorylation in DCs.

From: Optineurin modulates the maturation of dendritic cells to regulate autoimmunity through JAK2-STAT3 signaling

Fig. 6

a Expression of (p)-JAK2 in Ctrl or Optn deficient BMDCs stimulated with LPS for 8 h. b, c Ctrl and Optn deficient BMDCs were stimulated with LPS (b) or poly I:C (c) after transfection with empty or OPTN expression vector. The expressions of OPTN and p-JAK2 were confirmed by western blotting. d Western blotting for expression of (p)-JAK2 and (p)-STAT3 in Ctrl and Optn deficient BMDCs stimulated with anti-IL6 or anti-IL10 for 48 h. e Interaction between endogenous TLR4 and JAK2 or STAT3 in BMDCs was analyzed by IP with anti-JAK2 or anti-STAT3 using normal rabbit IgG as a control, followed by immunoblotting with anti-TLR4, anti-JAK2, and anti-STAT3. f Expression of (p-) signaling proteins in whole-cell lysates of Ctrl or Optn deficient BMDCs stimulated with LPS. g HEK293 cells were transfected with si-Optn or Optn plasmid before IL-6 stimulation. The expressions of OPTN and p-JAK2 were confirmed by western blotting. h Western blotting for expression of p-JAK2 and p-STAT3 in Ctrl and Optn deficient BMDCs stimulated with RAPA. All data are representative of three independent experiments. Source data are provided in Source data file.

Back to article page