Fig. 4: Trans-complementation of MAAP8 rescues secretion defect across multiple MAAPΔ AAV serotypes.
From: The membrane associated accessory protein is an adeno-associated viral egress factor
scCBh-GFP vectors produced with WT Cap or MAAPΔ Cap for AAV1 (A, B), AAV2 (C, D), AAV8 (E, F), and AAV9 (G, H) complemented in trans with a VP/AAP-null AAV8 plasmid to replicate endogenous levels of MAAP expression. Total vector genomes (A, C, E, G) and the proportion of vector found in the media and cells (B, D, F, H) 3 days post transfection. Each bar is a representation of three experiments that are biological replicates. Data are presented as mean values ± SD. Significance was determined by two-way ANOVA, with Tukey’s post-test. (AAV1 Cells vs AAV1 MAAPΔ Cells, ****p < 0.0001; AAV1 MAAPΔ Cells vs AAV1 MAAPΔ + MAAP8 Cells, ****p < 0.0001; AAV1 Total vs AAV1 MAAPΔ Total, ***p = 0.0002; AAV1 MAAPΔ Total vs AAV1 MAAPΔ + MAAP8 Total, *p = 0.0116; AAV8 Media vs AAV8 MAAPΔ Media, ****p < 0.0001; AAV8 Media vs AAV8 MAAPΔ + MAAP8 Media, *p = 0.0211; AAV8 MAAPΔ Media vs AAV8 MAAPΔ + MAAP8 Media, ****p < 0.0001; AAV8 Cells vs AAV8 MAAPΔ Cells, ****p < 0.0001; AAV8 MAAPΔ Cells vs AAV8 MAAPΔ + MAAP8 Cells, ****p < 0.0001; AAV8 Total vs AAV8 MAAPΔ Total, ****p < 0.0001; AAV8 Total vs AAV8 MAAPΔ + MAAP8 Total, ****p < 0.0001; AAV9 Media vs AAV9 MAAPΔ + MAAP8 Media, **p = 0.0071; AAV9 MAAPΔ Media vs AAV9 MAAPΔ + MAAP8 Media, *p = 0.0223; AAV9 Cells vs AAV9 MAAPΔ Cells, ***p = 0.0003; AAV9 MAAPΔ Cells vs AAV9 MAAPΔ + MAAP8 Cells, ***p = 0.0003;; AAV9 Total vs AAV9 MAAPΔ Total, ***p = 0.0001; AAV9 Total vs AAV9 MAAPΔ + MAAP8 Total, **p = 0.0075).
