Fig. 2: Analysis of fibroblast subsets. | Nature Communications

Fig. 2: Analysis of fibroblast subsets.

From: The serine proteases dipeptidyl-peptidase 4 and urokinase are key molecules in human and mouse scar formation

Fig. 2

A Phylogenetic clustertree calculated based on UMAP-clustering of subsetted fibroblasts only. B, C UMAP-plots of re-clustered skin and scar fibroblasts, split by tissue, reclustering identified 11 fibroblast clusters (subFB1-11). D Feature plots illustrating computational basis for dotplots. Dotplots of top 50 regulated genes (according to lowest adjusted p-value) comparing scar FBs versus skin FBs. E Dotplot of top 50 regulated genes (according to lowest adjusted p-value) cluster subFB1 compared to all other scar FBs. F Gene ontology-term network was calculated based on significantly upregulated (adj. p-value <0.05, avg.logFC >0.1) genes comparing subFB1 to all other scar FBs. Gene list was imputed in ClueGO plug-in in Cytoscape with medium GO-specificity, with GO-term fusion, only significant (P value < 0.05) GO terms are shown. Circle size correlates with P value, lines (“edges”) represent functional connection of respective GO terms. Red circles represent association of GO-term with TGFβ-signaling, purple, with extracellular matrix, green, with smooth muscle differentiation, blue, with signaling factors, and yellow with bone formation and -development. UMAP, uniform manifold approximation and projection.

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