Fig. 7: Inactivated NDV-HXP-S induces protective antibody response against the challenge of SARS-CoV-2 variants of concern. | Nature Communications

Fig. 7: Inactivated NDV-HXP-S induces protective antibody response against the challenge of SARS-CoV-2 variants of concern.

From: A Newcastle disease virus expressing a stabilized spike protein of SARS-CoV-2 induces protective immune responses

Fig. 7

Eight-to-ten-week-old female BALB/c mice were either vaccinated with 1 µg of inactivated NDV-HXP-S (n = 4) or WT NDV (n = 4, negative control). Two immunizations were performed via the intramuscular route at D0 and D21. At D44, mice were treated with Ad5-hACE2. At D49, one-third of mice from each group was challenged with USA-WA1/2020, B.1.351, or P.1 strain. On day 2, lungs were harvested and homogenized in 1 mL PBS. Viral titers were measured by plaque assay on Vero E6 cells and plotted as GMT of PFU/mL (LoD=50 PFU/mL; A titer of 25 PFU/mL was assigned to negative samples). The error bars represent geometric SD. Statistical difference was analyzed by a one-tailed t test. The p values are indicated.

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