Fig. 6: Structure of TphC showing ligand-induced changes.
From: Structural basis of terephthalate recognition by solute binding protein TphC
A TphC crystal structure in both an apo (open—orange) and TPA-bound (closed—blue) conformation have been superimposed (SSM superposition of domain 1) in ICM-Pro. The protein surfaces for each of the two states are shown as meshes and coloured according to their respective structure (open—orange, closed—blue, the surface representations correspond to thin slices through the surface centred on the TPA-binding site). Helical elements within the structure have been highlighted as large cylinders to clearly show the reorientation between domains 1 and 2 which occur upon TPA binding. TPA is shown in both ball and stick and semitransparent CPK spheres (all atom colours). B A ball and stick representation of the TPA-binding site along with highlighted hydrogen bonds that form both between the protein and the TPA and also between the protein domains upon TPA binding. Residues arising from domain 1 are shown with yellow carbon atoms while those from domain 2 are shown with grey carbon atoms.
