Fig. 7: Anotated phylogenetic tree of TphC and configuration of putative tph-like operons.
From: Structural basis of terephthalate recognition by solute binding protein TphC
A Phylogenetic tree of TphC constructed by retrieving, aligning and displaying sequences (using BLAST-P, Clustal Omega and iTOL respectively). Species names are coloured by taxonomic family (n = 12) and taxonomic class is shown by a coloured outer ring (α-proteobacteria (red) and β-proteobacteria (green). Genomic loci proposed to be tph-operon homologues are anotated (tph). Additionally, the amino acids (and their homologue equivalent) directly involved in ligand binding within the TphC-TPA are labelled, where P86, Q150, T155, and T242 (or A242) are labelled as PQTT (or PQTA). B Schematic diagram of the operon configuration for a selection of putative tph-like operons. Taxonomic family is shown by coloured boxes to the left of the operon. The values inside the genes indicate the percentage similarity of the encoded protein to the corresponding protein in Comamonas sp. strain E6. These operons show a range of alternative features, such as the presence of genes encoding transposases (Comamonas thiooxidans), a hydrolase (Ideonella sakaiensis), and alternative operon architectures (Roseomonas deserti and Bradyrhizobium ivorense).
