Fig. 3: Age-dependent changes in expression of Navβ3 and Navβ4 mRNAs in the arcopallium of male zebra finches.
From: Resurgent Na+ currents promote ultrafast spiking in projection neurons that drive fine motor control
a–d Representative in situ hybridization images for Navβ4 mRNA within RA across ages indicated by days post hatch (dph); black arrowheads indicate RA borders. e–h. Representative in situ hybridization images for Navβ3 mRNA within RA across ages. Black arrowheads indicate RA borders. i Comparison of Navβ4 expression levels (normalized optical density) across age groups within RA (black) and in a caudal arcopallial region of equal size outside RA (gray). Significant age differences were observed in RA (one-way ANOVA with Tukey’s post hoc; P = 0.005, F (3, 12) = 7.416, N = 4 males per age). Data are presented as individual data points with bars as mean values ± SEM. j Comparison of the proportions of Navβ4-expressing cells across age groups within RA (black) and in an arcopallial region of equal size outside RA (gray). Significant age differences in RA (one-way ANOVA with Tukey’s post hoc; P = 0.001, F = F (3, 12) = 10.79, N = 4 males per group). Data are presented as individual data points with bars as mean values ± SEM. k Comparison of Navβ3 expression level (normalized optical density) across age groups within RA (black) and in an arcopallial region of equal size outside RA (gray). Significant age differences in RA (one-way ANOVA with Tukey’s post hoc; P = 5.4 × 10−8, F (3, 12) = 73.43, N = 4 males per group). Data are presented as individual data points with bars as mean values ± SEM. l Comparison of the proportions of Navβ3-expressing cells across age groups within RA (black) and in an arcopallial region of equal size outside RA (gray). Significant age differences in RA (two-way ANOVA with Tukey’s post hoc; P = 1.6 × 10−8, F (3, 12) = 90.96, N = 4 males per group). Data are presented as individual data points with bars as mean values ± SEM.
