Fig. 8: Dynamic clamping of spiking RAPNs in juvenile and adult male finches.
From: Resurgent Na+ currents promote ultrafast spiking in projection neurons that drive fine motor control
a Family of recorded INaR (same as in Fig. 2c). b Family of modeled INaR currents (same voltage protocol that elicited currents in Fig. 2c; same scale as in (a)). c Overlay of the current-voltage relationship of peak INaR comparing the modeled (red) and average recorded values (black; same as in Fig. 2e). d Overlay of the time constant of decay (τ) as a function of voltage in the modeled (red) and recorded currents (black). e APs elicited in 20 days post hatch (dph) RAPNs before (OFF; black) or during dynamic clamp-mediated addition (ON; red) of the in silico INaR (blue) peaking during the AP repolarization phase. APs were triggered by a +300 pA current injection. f Paired data of the instantaneous firing frequency (Inst. Firing Freq.) change recorded in 20 dph RAPNs upon dynamic clamp-mediated addition (ON; pink) compared to the control (OFF; gray) condition as a function of conductance (mean ± SEM in black, two-tailed paired t test, P-values in graph; N = 8 RAPNs). g APs elicited in adult RAPNs before (OFF; black) or during dynamic clamp-mediated subtraction (ON; red) of the in silico INaR (blue) peaking during the AP repolarization phase. APs were triggered as in (e). h Paired data of the instantaneous firing frequency change recorded in adult RAPNs upon dynamic clamp-mediated subtraction (ON; pink) compared to the control (OFF; gray) condition as a function of conductance (mean ± SEM in black, two-tailed paired t test, P-values in the graph; N = 5 RAPNs).
