Fig. 5: Effect of Lsi1 mutation on the transport activity for Ge and As.
From: Structural basis for high selectivity of a rice silicon channel Lsi1
Transport activity of germanic acid (Ge) (a, Si analog) and arsenite (As) (b) in Xenopus oocytes. The oocytes were injected with water (as a control) or cRNA of Lsi1 WT or Thr65TM1 mutants. Oocytes were exposed to a solution containing Ge or As for 30 min. Transport activity of Ge (c, e) and As (d, f) in Sf9 cells. C-terminally EGFP-tagged Lsi1 or Thr181 mutants (c, d) or other aquaglyceroporins (e, f) were expressed in the cells. The cells were exposed to a solution containing Ge or As for 5 min. In a−f, different letters above the columns indicate statistically significant differences at P < 0.01 by Tukey−Kramer’s test, and the test was two-sided. Values are means ± s.d., n = 3 for T65C in (a) and n = 4 for the others, n is independent experiments.
