Fig. 8: Analysis of selectivity filters in NIP subfamilies.
From: Structural basis for high selectivity of a rice silicon channel Lsi1

Phylogenetic tree of plant NIP subfamilies (a), and summary of the residues comprising the selectivity filer and Thr181 (b). The phylogenetic tree was constructed using the neighbor-joining algorithm by the software MEGAX51 after the sequence alignment using Clustal Omega52 with 1,000 bootstrap trials. The 0.05 scale indicates substitution distance.