Fig. 2: Inhibition of rRNA biogenesis induced an expanded 2C-like cell population in mES cells. | Nature Communications

Fig. 2: Inhibition of rRNA biogenesis induced an expanded 2C-like cell population in mES cells.

From: rRNA biogenesis regulates mouse 2C-like state by 3D structure reorganization of peri-nucleolar heterochromatin

Fig. 2

a tSNE feature plots demonstrating the averaged expression levels of MERVL genes in 2981 control mES cells and 3219 CX-5461-treated mES cells. GEO accession code GSE166041. b tSNE feature plots demonstrating the averaged expression levels of ribosome genes in 2981 control mES cells and 3219 CX-5461-treated mES cells. GEO accession code GSE166041. c tSNE feature plots demonstrating the averaged expression levels of ribosome biogenesis genes in 2981 control mES cells and 3219 CX-5461-treated mES cells. GEO accession code GSE166041. d Scatter plots demonstrating negative correlation of expression level between MERVL/MERVL-int/MT2_Mm and ribosome genes; Each dot represents a single cell with detectable ERV expression; r denotes correlation coefficient; p-value was obtained by cor.test function in R software. GEO accession code GSE166041. e FACS analysis on 2C::tdTomato+ mES cells upon different treatment doses of CX-5461, showing the change of percentage of 2C-like cells. f FACS analysis on 2C::tdTomato+ mES cells after 12 h of treatment and 24 h withdrawal of CX-5461, showing the change of percentage of 2C-like cells.

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