Fig. 3: Deficiency of rRNA biogenesis disrupted the normal state of nucleolar LLPS.

a CX-5461 treatment causes abnormal nucleolar structure with electron microscopy; Experiment was repeated independently three times with similar results. b Immunofluorescence staining of NCL in control mES cells and CX-5461-treated mES cells; Experiment was repeated independently three times with similar results. c Immunofluorescence staining of NPM1 in control mES cells and CX-5461-treated mES cells; Experiment was repeated independently three times with similar results. d Immunofluorescence staining of FBL in control mES cells and CX-5461 treated mES cells; Experiment was repeated independently three times with similar results. e Immunofluorescence staining of RPA194 in control and CX-5461 treated mES cells; Experiment was repeated independently three times with similar results. f FRAP analysis showing CX-5461 treatment causes accelerated recovery after photobleaching of NCL; N = 3 biologically independent experiments; Data are presented as mean values +/− SEM. SEM standard error of mean. Shown images are representative of three times of biologically independent experiments. g FRAP analysis showing CX-5461 treatment causes accelerated recovery after photobleaching of NPM1; N = 3 biologically independent ChIP-seq experiments; Data are presented as mean values +/− SEM. SEM standard error of mean. Shown images are representative of three times of biologically independent experiments. h FRAP analysis showing CX-5461 treatment causes accelerated recovery after photobleaching of FBL; N = 3 biologically independent ChIP-seq experiments; Data are presented as mean values +/− SEM. SEM standard error of mean. Shown images are representative of three times of biologically independent experiments. Source data are provided as a Source Data file.