Fig. 5: Multi-cell layer model for the regulation of the WUS protein gradient. | Nature Communications

Fig. 5: Multi-cell layer model for the regulation of the WUS protein gradient.

From: CLAVATA3 mediated simultaneous control of transcriptional and post-translational processes provides robustness to the WUSCHEL gradient

Fig. 5

a The WUS-CLV3 feedback loop model with WUS-mediated concentration-dependent regulation of CLV3 and CLV3-mediated dual transcriptional and post-translational regulation of WUS. b Model simulation of nuclear WUS protein, cytoplasmic WUS protein, and WUS expression in wild type and clv3-2 mutant SAMs. c Ratio of L3 to L1 (L3:L1) nuclear WUS (mean ± s.d.) for wild type and clv3-2 mutants. For experimental quantification independently imaged wild type (n = 7) and clv3-2 (n = 8) SAMs were considered. For simulations “n” represents the number of cells in wild-type L1 [n = 8] and L3 [n = 6], and in clv3-2 L1 [n = 8] and L3 [n = 6]). d Model prediction of nuclear export rates across cell layers (L1 [n = 8], L2 [n = 7], and L3 [n = 6], mean ± s.d.) of wild type and clv3-2 mutants. The “n” represents the number of cells considered for quantification. e Transcriptional and post-translational experimental support in the computational model. f Simplified model of transcriptional and post-translational regulation of WUS.

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