Fig. 6: Dynamics of WUS-CLV3 feedback in simulations with CLV3-mediated transcriptional regulation, dual transcriptional and post-translational regulation, and dual regulation with the spatial requirement of CLV3 expression from the outer layers, upon perturbing the WUS expression rate.

a In the transcriptional regulation model, high WUS concentration represses CLV3, which relieves WUS repression leading to more WUS production. b Low WUS concentration in the transcriptional regulation model activates CLV3 which continues to suppress WUS expression, lowering WUS levels. c In contrast in the CLV3-mediated dual regulation model, high WUS leads to low CLV3 which leads to increased nuclear export decreasing the nuclear concentration and increased WUS synthesis generating new WUS molecules. d Low WUS levels in the dual regulation model with the spatial requirement of CLV3 expression from the outer cell layers leads to internalization of CLV3 that is less potent in repressing WUS and reducing nuclear export which leads to increased synthesis of diffusible cytoplasmic WUS to restore WUS levels in the outer layers. With WUS levels restored, CLV3 expression gets restored back to the outer layers which in turn reduces the nuclear export of WUS in the outer layers to restore WUS production and the nuclear WUS concentration. e–g WUS expression (brown), the WUS nuclear protein gradient (green), and CLV3 expression (yellow) in the SAM upon perturbations in WUS expression level in simulated e CLV3-mediated transcriptional regulation model, f CLV3-mediated dual transcriptional and post-translational regulation model, and g CLV3-mediated dual regulation with the spatial requirement of CLV3 expression from outer layers model.