Fig. 2: Intracellular RBM20 localization in RBM20 Mutant iPSC-CMs. | Nature Communications

Fig. 2: Intracellular RBM20 localization in RBM20 Mutant iPSC-CMs.

From: Gain-of-function cardiomyopathic mutations in RBM20 rewire splicing regulation and re-distribute ribonucleoprotein granules within processing bodies

Fig. 2

a Immunofluorescence for RBM20 in WT and RBM20 mutant iPSC-CMs. Spinning disk confocal, scale bar: 5 μm. Representative micrographs from n > 3 experiments. b Structured illumination microscopy of DNA (magenta) and RBM20 (green). Left WT image represents X–Y maximum intensity projection, right images represent X–Z 3D projections. Yellow arrowheads indicate perinuclear localization of RBM20 in mutant cells. Yellow stars indicate the nuclear localization of RBM20. Scale bar: 5 μm. Representative micrographs from n > 3 experiments. c 3D localization analysis of RBM20 from 3D-SIM images. Magenta bars (left bar above genotype) indicate percent nuclear localization, green bars (right bar above genotype) indicate percent cytoplasmic localization. Data represents per-cell averages from N = 2 independent experiments with n = 4 cells per genotype. Data represented as mean values ± SEM.

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