Fig. 5: The effects of RPA and CST on RAD51-mediated strand exchange.
From: Crosstalk between CST and RPA regulates RAD51 activity during replication stress
a Schematic of the DNA strand-exchange assay. The 32P-labeled DNA is marked by an asterisk. b, c RPA (b) or CST (c) was preincubated with ssDNA at the indicated concentrations of KCl. RAD51 activity was determined by monitoring the formation of strand-exchange products. Note that 5 mM CaCl2 was included in the reaction to stimulate RAD51 activity. d, e The indicated amounts of RPA and CST were preincubated with ssDNA and 150 mM KCl before adding RAD51. CaCl2 (d) or AMPPNP (e) was used to stimulate RAD51 activity. b–e Quantitative plots are shown below the gel images. Data represent mean ± S.D. calculated from three independent experiments. NS not significant, **P < 0.01; ***P < 0.001; ****P < 0.0001, as calculated by one-way ANOVA with Tukey’s post hoc test.
