Fig. 2: The nuclear inclusions formed by Httex1 72Q do not exhibit the classical core and shell organization observed for the cytosolic inclusions. | Nature Communications

Fig. 2: The nuclear inclusions formed by Httex1 72Q do not exhibit the classical core and shell organization observed for the cytosolic inclusions.

From: Nuclear and cytoplasmic huntingtin inclusions exhibit distinct biochemical composition, interactome and ultrastructural properties

Fig. 2

a Representative confocal images of Httex1 72Q nuclear inclusions, 48 h after transfection. Httex1 expression (grey) was detected using a specific primary antibody against the N-terminal part of Htt (amino acids 1-17; 2B7 or Ab109115). The nucleus was counterstained with DAPI (blue), and phalloidin (red) was used to stain the actin F. Httex1 nuclear inclusions are indicated by the yellow arrowheads. Scale bars = 10 μm. b Electron micrograph of a representative nuclear Httex1 72Q inclusion. The white square indicates the area shown at higher magnification in the right-hand panel. The nucleus is highlighted in blue, and the double arrows indicate the distance between the nuclear inclusion and the nuclear membrane. No interaction between the nuclear inclusion and the nuclear membrane was observed. Scale bars = 2 μm (left-hand panel) and 500 nm (right-hand panel).

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