Fig. 6: Ultrastructural analysis of the nuclear Httex1 72Q inclusions formed in primary cortical neurons shows granular and filamentous structures by CLEM and Tomography. | Nature Communications

Fig. 6: Ultrastructural analysis of the nuclear Httex1 72Q inclusions formed in primary cortical neurons shows granular and filamentous structures by CLEM and Tomography.

From: Nuclear and cytoplasmic huntingtin inclusions exhibit distinct biochemical composition, interactome and ultrastructural properties

Fig. 6

a Representative electron micrographs of Httex1 72Q inclusions formed 7 days after lentiviral transduction in mouse cortical primary neurons. Blue dashed lines represent the inclusions, and the red arrowheads the nucleolus. Scale bars = 500 nm for the EM images and 50 μm for the fluorescent image (inset). b Electron micrograph and corresponding tomogram (selected area in red) of a neuronal Httex1 72Q inclusion in panel a. Segmentation of the tomogram reveals the presence of a multitude of filaments. c Representative electron micrographs of ΔNt17 Httex1 72Q inclusions formed seven days after lentiviral transduction in mouse cortical primary neurons. Orange dashed lines represent the inclusions, and the red arrowheads the nucleolus. Scale bars = 500 nm for the EM images and 50 μm for the fluorescent image (inset).

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