Table 1 Primers used for detecting exon skipping.

From: Low immunogenicity of LNP allows repeated administrations of CRISPR-Cas9 mRNA into skeletal muscle in mice

 

Unskipped

Skipped

Standard DNA

5′-AGCAATCCTCAAAAACAGATGCCAGTATTCTACAGGAAAAATTGGGAAGCCTGAATCTGCGGTGGCAGGAGGTCT-3′

5′-GAAGCCGTGGCACAGATGGATTTCCAGGGGGAAAAACTTCATAGAATGTACAAGGAACGACAAGGGATTGAAGAACAAAAGAATGTCT-3′

Forward primer

5′-TCCTCAAAAACAGATGCCAGTATTC-3′

5′-CGTGGCACAGATGGATTTCC-3′

Reverse primer

5′-TCCTGCCACCGCAGATTC-3′

5′-TTCTTTTGTTCTTCAATCCCTTGTC-3′

FAM-probe

5′-ACAGGAAAAATTGGGAAGC-3′

5′-ACTTCATAGAATGTACAAGGAA-3′