Table 2 Primers and probes for ddPCR.

From: Low immunogenicity of LNP allows repeated administrations of CRISPR-Cas9 mRNA into skeletal muscle in mice

 

Non target site

Target site

Forward primer

5′-GACATGCCCATATCCAAAGGA-3′

5′-TTTGCCGCTGCCCAAT-3′

Reverse primer

5′-AACCGAGAGGGTGCTTTTTTC-3′

5′-CATTTTTGTTTTGCCTTTTTGGT-3′

Probe

5′-ACAAGACAGAAAGACACCTT-3′

5′-CCATCCTGGAGTTCC-3′

VIC labeled

FAM labeled

Back to article page