Fig. 1: Analysis of the 19Q4 Avana dataset from the DepMap initiative.

a Number of screened cell lines in each of the 26 lineages represented in the dataset. b Left, number and percentage of gRNAs in the Avana library that have 0, 1, or more than 1 perfect targets (H0) in the human genome (hg38 assembly) or that have specificities (s) lower or equal/higher than 0.16. Right, number of perfect target sites for gRNAs with H0 > 1. The gRNA in the Avana library with the highest number of perfect targets sites is highlighted. c–d Summary of the number (c) and percentage (d) of genes targeted by increasing number of promiscuous gRNAs (1-6+). Promiscuity is defined at three distinct thresholds (H0 > 1, s < 0.10, s < 0.16). e Specificity scores of all gRNAs (n = 108,345) with varying numbers of perfect target sites in the genome. X-axis is truncated at 31 for simplicity. Dashed line highlights the position of the 0.16 threshold. f Specificity scores of gRNAs with varying number of perfect (left; n = 2423) or single mismatched (right; n = 2374) targets in the genome in the absence of any additional Hamming neighbors. gRNAs with no perfect target in the hg38 genome were excluded from the analysis in e and f. All boxplots show minimum, maximum, median, first, and third quartiles.