Fig. 4: Identification of a CN-RtTg direct projection.
From: A Brainstem reticulotegmental neural ensemble drives acoustic startle reflexes
a Schematic for the anterograde tracing from CN to RtTg. b Representative images showing CN injection site and EGFP+ presynaptic terminals in RtTg. Scale bar, 200 μm. c Quantitative fluorescence intensity of CN EGFP+ presynaptic terminals in the ipsilateral and contralateral RtTg (n = 15; P = 8.87 × 10−9). d Schematic for the anterograde transsynaptic tracing from CN to RtTg. e Representative images showing CN injection site and the tdT+ cells in RtTg. Scale bar, 200 μm. f Quantification of tdT+ cell numbers in the ipsilateral and contralateral RtTg (n = 8; P = 3.8 × 10−7). g Left: a representative image showing tdT+ RtTg neurons co-labelled with NeuN (white arrowheads). Scale bar, 50 μm. Right: pie chart indicating the percentage of NeuN+ neurons co-labelled with or without tdT. h Schematic for the retrograde tracing from RtTg to CN. i Representative images showing EGFP+ neurons in ipsilateral and contralateral CN. Scale bar, 200 μm. j Quantification of EGFP+ cells number in the ipsilateral and contralateral CN (n = 8; P = 5.09 × 10−6). Error bar represents mean ± s.e.m. Significance was assessed using two-sided paired t-tests in c, f, j. ***P < 0.001.
