Fig. 3: Experimental comparisons on the histone-labeled Drosophila embryo with high-speed axial scanning. | Nature Communications

Fig. 3: Experimental comparisons on the histone-labeled Drosophila embryo with high-speed axial scanning.

From: Computational optical sectioning with an incoherent multiscale scattering model for light-field microscopy

Fig. 3

a, b 3D rendered volumes and 180-μm-xz maximum intensity projections (MIP) at the same time point obtained by traditional LFM and QLFM with a 40×/1.0NA water-immersion objective at an axial step of 15 μm. QLFM shows much better contrast and resolution than traditional LFM without artifacts, illustrating the capability of computational optical sectioning by QLFM with the improved penetration depth. c Depth-coded MIP of a Drosophila embryo reconstructed by traditional LFM and QLFM. d Slices reconstructed by different methods at various time stamps. e, f The comparisons between traditional LFM and QLFM under a ×20/0.5NA objective in the form of 3D rendered volumes and orthogonal MIPs with axial scanning at a step of 50 μm, indicating the uniform resolution of QLFM without edge artifacts. The images were processed with Volren modules in Amira 5.4 to get the volume renderings with similar contrast. Scale bars, 50 μm (a–d) and 100 μm (e, f).

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