Fig. 3: α-1,3-glucan is present in both alkali-soluble and insoluble fractions of the parental sample.
From: A molecular vision of fungal cell wall organization by functional genomics and solid-state NMR
a 1D 13C CP spectra detecting the rigid molecules in the alkali-soluble (AS) and alkali-insoluble (AI) fractions of the parental sample. b Overlay of 2D 13C CP dipolar-INADEQUATE spectra showing the rigid molecules in the alkaline-soluble (AS, orange) and alkaline-insoluble (AI, black) portions of A. fumigatus cell walls. Signals of α-1,3-glucans, such as A4, A5, and A6, are present in both portions. c 13C DP J-INADEQUATE spectrum showing the mobile polysaccharides in the alkali insoluble (left) and soluble (right) parts. d Compositional analysis of both AI (blue) and AS (orange) fractions obtained by GC-HPLC and enzymatic degradation. The x-axis reports different monosaccharide units as well as the amino acids (AA) or valine (Val). e Relative mass percentages of the AI and AS fractions. f Relative fractions of β-1,3-glucan and α-1,3-glucan. g Summary of polysaccharides and proteins identified in both rigid and mobile portions within the alkali insoluble and soluble fractions. Source data of (d–f) are provided as a Source Data file.
