Fig. 4: CD4-Cre mediates conditional deletion of SHP2 in chondrocytes.
From: Targeting chondrocytes for arresting bony fusion in ankylosing spondylitis
Sagittal section of joints from CD4-CKO; Rosa26-mTmG mice were immunostained with marker protein GFP, transcriptional regulator SOX9 and nuclear counterstain DAPI. a, b Immunofluorescence staining images show SOX9 expression in all chondrocytes and GFP expression in hypertrophic chondrocytes in knee and spine section of P3 CD4-CKO; Rosa26-mTmG mice. a Blue dotted box shows cartilage mold, and green dotted box indicates hypertrophic zone of the growth plate. c, d Representative images of 1-month-old CD4-CKO; Rosa26-mTmG mice show GFP+ chondrocytes consisted in hypertrophic zone of femur and spine. e, f Immunofluorescence analysis of articular cartilage and growth plate (e) and chondroma (f) of the tibia in 7-month-old CD4-CKO; Rosa26-mTmG mice show GFP+ differentiated chondrocytes in the growth plate and articular cartilage of mature CD4-CKO;Rosa26-mTmG mice. e The area inside the two white dotted lines is articular cartilage (AC) (upper) and growth plate (GP) (under). a–f Scale bars, 100 μm. Data are representative of three independent experiments.
