Fig. 3: Hyperglutamatergic function of Drd2-positive neurons in SR-Drd2+/− rats.
From: Dopamine D2 receptor regulates cortical synaptic pruning in rodents
a Diagram to show the whole-cell recording of Drd2-positive neurons in layer 5 of ACC from SR-Drd2 rats. Left, the fluorescent image, right, the phase-contrast image. Scale bar, 10 μm. Three independent experiments were repeated to get similar results. b Representative mEPSC traces of Drd2-positive neurons from 4-week-old control and SR-Drd2+/− rats. c Similar mEPSC frequency of Drd2-positive neurons between 4-week-old control and SR-Drd2+/− rats. NS not significant, P = 0.9421, two-sided t-test, n = 14 neurons from 3 control rats, n = 13 neurons from 3 SR-Drd2+/− rats. Data are presented as mean values ± SEM. d Cumulative plots of mEPSC frequency. e Similar mEPSC amplitude of Drd2-positive neurons between 4-week-old control and SR-Drd2+/− rats. NS not significant, P = 0.5763, two-sided t-test, n = 14 neurons from 3 control rats, n = 13 neurons from 3 SR-Drd2+/− rats. Data are presented as mean values ± SEM. f Cumulative plots of mEPSC amplitude. g Representative mEPSC traces of Drd2-positive neurons from 5-week-old control and SR-Drd2+/− rats. h Increased mEPSC frequency of Drd2-positive neurons in 5-week-old SR-Drd2+/− rats. *P = 0.0435, two-sided t-test, n = 12 neurons from 3 control rats, n = 20 neurons from 4 SR-Drd2+/− rats. Data are presented as mean values ± SEM. i Cumulative plots of mEPSC frequency. j Similar mEPSC amplitude of Drd2-positive neurons between 5-week-old control and SR-Drd2+/− rats. NS not significant, P = 0.263, two-sided t-test, n = 12 neurons from 3 control rats, n = 20 neurons from 4 SR-Drd2+/− rats. Data are presented as mean values ± SEM. k Cumulative plots of mEPSC amplitude. l Representative mEPSC traces of Drd2-positive neurons from 8-week-old control and SR-Drd2+/− rats. m Increased mEPSC frequency of Drd2-positive neurons in 8-week-old SR-Drd2+/− rats. *P = 0.0139, two-sided t-test, n = 14 neurons from 3 control rats, n = 18 neurons from 4 SR-Drd2+/− rats. Data are presented as mean values ± SEM. n Cumulative plots of mEPSC frequency. o Similar mEPSC amplitude of Drd2-positive neurons between 8-week-old control and SR-Drd2+/− rats. NS not significant, P = 0.2503, two-sided t-test, n = 14 neurons from 3 control rats, n = 18 neurons from 4 SR-Drd2+/− rats. Data are presented as mean values ± SEM. p Cumulative plots of mEPSC amplitude. q Representative action potentials of Drd2-positive neurons from 8-week-old control and SR-Drd2+/− rats. r Increased excitability of Drd2-positive neurons in 8-week-old SR-Drd2+/− rats, compared with control rats. Shown are the I/O curves of action potentials from Drd2-positive neurons. *** Genotype F (1, 2010) = 57.55, P < 0.0001, two-way ANOVA, n = 25 neurons from 5 control rats, n = 44 neurons from 7 SR-Drd2+/− rats. Data are presented as mean values ± SEM.
