Fig. 8: Hyperglutamatergic function of Drd2-positive neurons after inhibition of DRD2 during adolescence.
From: Dopamine D2 receptor regulates cortical synaptic pruning in rodents
a Experimental design. SR-Drd2 rats received daily injection of Eti (1 μg in 0.5 μl per side) or Veh (0.5 μl saline per side) into layer 5 of ACC between 3- and 4-week-old age, the sEPSC, sIPSC, and cell excitability of Drd2-positive neurons were then analyzed at 8-week-old age. b Representative sEPSC traces of Drd2-positive neurons from 8-week-old Eti and Veh-treated rats. c Increased sEPSC frequency of Drd2-positive neurons in Eti-treated SR-Drd2 rats, compared with controls. *P = 0.0324, two-sided t-test, n = 14 neurons from 3 control rats, n = 15 neurons from 3 Eti-treated rats. Data are presented as mean values ± SEM. d Cumulative plots of sEPSC frequency. e Similar sEPSC amplitude of Drd2-positive neurons between Veh and Eti- treated rats. NS not significant, P = 0.0672, two-sided t-test, n = 14 neurons from 3 control rats, n = 15 neurons from 3 Eti-treated rats. Data are presented as mean values ± SEM. f Cumulative plots of sEPSC amplitude. g Representative sIPSC traces of Drd2-positive neurons from 8-week-old Veh and Eti-treated rats. h Similar sIPSC frequency of Drd2-positive neurons between Veh and Eti-treated rats. NS not significant, P = 0.9176, two-sided t-test, n = 16 neurons from 3 control rats, n = 22 neurons from 3 Eti-treated rats. Data are presented as mean values ± SEM. i Cumulative plots of sIPSC frequency. j Similar sIPSC amplitude of Drd2-positive neurons between Veh and Eti-treated rats. NS not significant, P = 0.5628, two-sided t-test, n = 16 neurons from 3 control rats, n = 22 neurons from 3 Eti-treated rats. Data are presented as mean values ± SEM. k Cumulative plots of sIPSC amplitude. l Representative action potentials of Drd2-positive neurons from 8-week-old Veh and Eti-treated rats. m Increased excitability of Drd2-positive neurons in Eti-treated rats, compared with controls. Shown are the I/O curves of action potentials from Drd2-positive neurons. * Genotype F (1, 62) = 5.933, P = 0.0177, two-way ANOVA, n = 31 neurons from 4 control rats, n = 33 neurons from 4 Eti-treated rats. Data are presented as mean values ± SEM.
