Fig. 4: Profiling stromal and immune cell populations in LUAD progression.
From: Deciphering cell lineage specification of human lung adenocarcinoma with single-cell RNA sequencing
a UMAP visualization of endothelial cell clusters, color-coded by identified cell subtypes. ECs: endothelial cells; EPCs: endothelial progenitor cells. b Heatmap of selected marker genes in EC cluster subtypes. c Top enriched pathway of ECs isolated from normal or tumor tissues as determined by GSEA. d UMAP visualization of fibroblast clusters, color-coded by identified cell subtypes. e Heatmap of selected marker genes in the fibroblast cluster subtypes. f Top enriched pathway of fibroblasts isolated from normal or tumor tissues as determined by GSEA. g UMAP visualization of lymphocyte clusters, color-coded by identified cell subtypes. h Heatmap of selected marker genes in the lymphocyte cluster subtypes. i UMAP visualization of myeloid cell clusters, color-coded by identified cell subtypes. j Heatmap of selected marker genes in the myeloid cell cluster subtypes. k Co-immunofluorescence staining for ECs (CD31, red), immune cells (CD45, gray) and fibroblasts (fibronectin, green) in normal and tumor tissues from different stages. Nuclei were stained blue (DAPI). Each staining in panels come from three samples. Scale bars: 20 μm. GSEA: Gene Set Enrichment Analysis; UMAP: Uniform Manifold Approximation and Projection.
