Fig. 1: Distinct leukemia clones expanded in extramedullary organs.

a Clonal abundance was assessed in different tissues and organs and mapped to single-cell RNA sequencing data. b The fractions of mouse cells, non-barcoded human leukemia cells (GFP−), and barcoded human leukemia cells (GFP+) in the mononuclear cells (MNC) of the peripheral blood from a xenografted mouse over time. c–e Representative images showing extramedullary sites of leukemia expansion. Quarters are shown in (d) as size references. Additional mice are shown in Supplementary Fig. 4. f–h Clonal distribution across different tissues and organs in representative mice. Each color represents one distinct genetic barcode corresponding to a leukemia clone. Additional mice are shown in Supplementary Figs. 5 and 6c, f. i Comparison of clonal abundance between the peripheral blood and the spleen or the ovary. Markers of the same shape represent data from one mouse. 99% confidence intervals of correlation were determined by the blood and spleen comparison and highlighted by dashed gray lines. j The CMC2 gene was significantly upregulated in clones more abundant in the ovary than clones more abundant in the blood. The black bar indicates the mean, and the white dot represents the median. Source data are provided as a Source Data file.