Fig. 4: Comparing the same leukemia clones during different chemotherapy treatments across multiple mice. | Nature Communications

Fig. 4: Comparing the same leukemia clones during different chemotherapy treatments across multiple mice.

From: Deciphering intratumoral heterogeneity using integrated clonal tracking and single-cell transcriptome analyses

Fig. 4

a Kaplan–Meier survival plots of PDX mice under various treatments. Color bars illustrate the duration of the treatment for each group. b Human chimerism in the peripheral blood. Shown are the means of all experimental mice. Mouse variations are provided in Supplementary Fig. 16. c Clonal abundance in mouse blood before treatments and at the end time point for each mouse. Each column represents one mouse. Each color represents one distinct genetic barcode corresponding to a leukemia clone. d, e Representative clonal dynamics during combination therapy in two PDX mice. Additional mice are shown in Supplementary Figs. 17–19. The black-colored clone in multiple mice of (c, d) and Supplementary Fig. 18 represents non-barcoded (GFP−) human cells from patient ALL06. PCR analysis reveals that these GFP− human cells did not carry any genetic barcode (Supplementary Fig. 20). They may have originated from a clone that had lost the barcode construct or that may have been collected due to a sorting error. These non-barcoded human cells were not detected in mice that received the vehicle treatment or short intensive therapy, or in any mice that received sorted GFP+ B-ALL cells derived from the other patient samples. Source data are provided as a Source Data file.

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