Fig. 5: Some B-ALL clones respond differentially to intensive and maintenance chemotherapy in distinct but consistent manners across multiple mice.

a Comparing chemotherapy response of the same clones in different tissues. Shown are the log₂ fold differences between the average clonal abundances of two chemotherapy treatments for each tissue. R Pearson’s correlation coefficient, P two-tailed P value. Each dot represents one clone. b Example clones that responded differently under various chemotherapy treatments. Each marker represents data from one tissue and one mouse. c Left, ALL04 clones that responded better to combination therapy than to intensive therapy. d Left, ALL04 clones that responded better to maintenance therapy than to intensive therapy. e Left, ALL20 clones that responded better to intensive therapy than to maintenance therapy. b–e P values were calculated by a two-sided Kruskal Wallace test with Bonferroni correction. c–e Right, genes significantly differentially expressed in the clones shown on the left (blue) compared to all other clones from the same patient samples (orange). The black bar indicates the mean, and the white dot represents the median. BM bone marrow, MNC mononuclear cells. P values were calculated by the one-sided Mann–Whitney U-test and adjusted using both the experimental data and the scramble data, see the Methods section for detail. Source data are provided as a Source Data file.